Laser capture microdissection under a microscope using a software interface a tissue section typically 5 50 micrometres thick is viewed and individual cells or clusters of cells are identified either manually or in semi automated or more fully automated ways allowing the imaging and then automatic selection of targets for isolation. Laser microdissection lmd allows the isolation of individual cells or chromosomes and is a well established technique for sample preparation prior to downstream analysis of the nucleic acid content via pcr or sequencing techniques here we describe the successful combination of the leica lmd system and qiagen kits for purification of nucleic acids even from small amounts. Highly enriched populations of normal or tumour associated stroma from the specimen were identified and isolated according to the manufacturer s protocol.
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